Poly-3-Hydroxybutyrate Functionalization with BioF-Tagged Recombinant Proteins
نویسندگان
چکیده
منابع مشابه
Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in recombinant Escherichia coli grown on glucose.
A recombinant Escherichia coli strain has been developed that produces poly(3-hydroxybutyrate-co-4-hydroxybutyrate) when grown in complex medium containing glucose. This has been accomplished by introducing into E. coli DH5 alpha separate plasmids harboring the polyhydroxyalkanoate (PHA) biosynthesis genes from Ralstonia eutropha (formerly named Alcaligenes eutrophus) and the succinate degradat...
متن کاملMicrobial degradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in soils.
The microbial degradation of tensile test pieces made of poly(3-hydroxybutyrate) [P(3HB)] or a copolymer of 90% 3-hydroxybutyric acid and 10% 3-hydroxyvaleric acid was studied in soils incubated at a constant temperature of 15, 28, or 40 degrees C for up to 200 days. In addition, hydrolytic degradation in sterile buffer at temperatures ranging from 4 to 55 degrees C was monitored for 98 days. D...
متن کاملEffect of Carbon Nanotube Functionalization on the Structure and Properties of Poly(3- Hydroxybutyrate)/mwcnt Biocomposites
Poly(3-hydroxybutyrate) (PHB) is a natural, linear, thermoplastic polyester synthesized by microorganisms as intracellular carbon reserves and ion sinks in response to limited nutrient availability and imposed stress conditions. The PHB has become one of the most interesting biomaterials for application in tissue engineering due to its excellent biodegradability and biocompatibility. Nanocompos...
متن کاملAlternative block polyurethanes based on poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and poly(ethylene glycol).
A series of amphiphilic alternative block polyurethane copolymers based on poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P3/4HB) and poly(ethylene glycol) (PEG) were synthesized by a coupling reaction between P3/4HB-diol and PEG-diisocyanate, with different 3HB, 4HB, PEG compositions and segment lengths. Stannous octanoate was used as catalyst. The chemical structure, alternative block arrangem...
متن کاملFed-Batch Synthesis of Poly(3-Hydroxybutyrate) and Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate) from Sucrose and 4-Hydroxybutyrate Precursors by Burkholderia sacchari Strain DSM 17165
Based on direct sucrose conversion, the bacterium Burkholderia sacchari is an excellent producer of the microbial homopolyester poly(3-hydroxybutyrate) (PHB). Restrictions of the strain's wild type in metabolizing structurally related 3-hydroxyvalerate (3HV) precursors towards 3HV-containing polyhydroxyalkanoate (PHA) copolyester calls for alternatives. We demonstrate the highly productive bios...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2018
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.02595-17